High Dose 5 Day Cardarine Cycle Does Not Cause Cancer In Mice

Oh great, this fucking subject again. Haven’t we beaten this to death already a million times before?

This is just a quick update.

/u/nevercomment broke his vow of silence about a month ago to share this study (sorry it took so long to get to it bro). The study is a good read if you have some time (took my dumb ass a few days of re-reading it) to properly break it down and understand each term they are referencing, but the summary on page 1 is sufficient for our needs. I am going to break it up and paraphrase sections for our sake:

PPAR’s, such as cardarine, increase skeletal muscle fatty acid catabolism, improve insulin sensitivity, increase HDL (‘good cholesterol’), elicit anti-inflammatory activity and induce terminal differentiation (where cells become specialized) - all positive traits not typically found in a compound that cause tumor growth. However, as we all know, there are findings that PPARb/d ligands (cardarine) increase or support tumor growth by increasing the rate at which cells proliferate (by inhibiting apoptosis through phosphorylation of Akt and by increasing cyclooxygenase-2 (COX2) and vascular endothelial growth factor (VEGF) expression).

The contradictory findings could be due to differences in the model system (cancer cell line versus in vivo), differences in cell culture conditions (with and without serum) or differences in ligands. This study examined the effect of two different compounds (GW0742 and GW501516 aka cardarine) in human cancer cell lines cultured in the presence or absence of serum and compared in vitro (in a culture dish) analysis with in vivo (in a live organism) analysis.

Neither GW compound increased cell growth or phosphorylation of Akt and no increase in the expression of VEGF or COX2 were detected in any cancer cell line in the presence or absence of serum. Similarly, liver, colon and colon polyps from mice administered these compounds did not exhibit changes in these markers.

So essentially, we have a study that is at odds with other studies done, probably because of the dosing. Dose was 10mg/kg daily for 5 days, or about 160mg for a 100kg male. My personal conclusion is that this does not call into question the original studies that show the incidence of cancer, but reaffirms that length of time running this compound can not be ignored. A 5 day long cycle at a very high dose was not sufficient to cause cancer, whereas running the compound for 2 years does.